Services

Services Provided

TECHNICAL EXPERTISE

CMMCA investigators bring broad expertise across several disciplines including research in environmental engineering and remediation, geomicrobiology and mineral production, biogeochemistry and nutrient cycling, community ecology and assembly, gene expression, and molecular evolution.

DNA EXTRACTION

The CMMCA is staffed with individuals experienced with extracting DNA from bacterial and fungal pure cultures as well as environmental samples such as soil, wastewater, and crude oil.

DNA AND RNA QUANTIFICATION

The CMMCA provides services to quantify DNA and RNA extracts using the Qubit Fluorometer.

FLUORESCENCE IN SITU HYBRIDIZATION (FISH)

The CMMCA provides services for carrying out FISH analysis to detect the presence of ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, phosphate-accumulating organisms, and glycogen-accumulating organisms.

QUANTIFICATION OF SPECIFIC FUNCTIONAL GENE THROUGH QPCR

The CMMCA possesses the necessary facilities to carry out qPCR on DNA samples to quantify specific functional genes.

NEXT-GENERATION SEQUENCING

The CMMCA also provides a comprehensive Illumina Next-Generation library prep and sequencing service. Our staff is experienced in generating Illumina sequencer-compatible libraries from bacterial and/or fungal RNA as well as from DNA with high or low yields. The CMMCA The customer has the option of sequencing generated libraries on either an Illumina Hi-Seq 2500 or MiSeq sequencer.

DATA ANALYSIS

The CMMCA provides data analysis services for analyzing metagenomic sequence data generated through Illumina Next-Gen sequencing of environmental samples. The CMMCA utilizes the Advanced Computing Facility at KU to analyze metagenomic sequence data in a short period of time.